Isolation Of Compounds Of Steroids Teripang Gamat (Stichopus variegatus) With Various Types Of Solvents

Sea cucumber is one of the fisheries commodity that has an important economic value. Generally is<br />traded in dried form (beche-de-mer). One of thebioactive substances contained in sea cucumber is steroid<br />compounds that serves as an aphrodisiac and sex reversal. The purpose of this study was to extract the<br />steroid of the gamma sea cucumber by using three types of solvents (methanol, ethyl acetate and hexane)<br />and get the best solvent in producing the highest yield of the steroids. The study revealed that steroid of<br />gamma sea cucumber (Stichopus variegatus) dissolved completely ethyl acetate (semi-polar solvent) during<br />the first phase, second phase and the third phase of extraction. In the methanol (polar solvent) steroids only<br />dissolved in the first extraction phase, while using the hexane (non polar solvent) steroid was undetectable.<br />Fractionation by thin layer chromatography was obtained two fractions that identified as cholesterol (Rf =<br />0.96) and testosterone (Rf = 0.91).<br />