PEMANFAATAN ANTIBODI DALAM DIAGNOSIS DEMAM BERDARAH DENGUE

<p>The Use of Antibody For Dengue Hemorrhagic Fever Diagnosis.</p><p>Dengue Hemorrhagic Fever (DHF) is caused by a virus belongs to Flavivirus group; the diseaseis transmitted by mosquito bites (Ae. aegyti and Ae. albopictus). There are four dengue virus serotypes (Dengue-1, Dengue-2, dengue-3 and Dengue-4), these all 4 serotypes were found in Indonesia. The majority of the mortality cases were due to the serious manifestation of the infections, such as DHF and Dengue Shock Syndrome (DSS). In general, the case fatality rates (CFR) of the disease are about 5 % for DHF and 40% for DSS. ln Indonesia 2006, even though the morbidity was increased, but the CFR was decreased. However, the CFR is still higher than the national control program target which is &lt; 1%. The symptom of illnesses are very non specific as flu-like mild undifferentiated fever, it was hard to differentiate the fever of dengue from others. Diagnosis of dengue virus infection on the basis of clinical syndromes is not reliable, and the diagnosis should be confirmed by laboratory studies. Laboratory diagnosis of dengue virus infection can be made by the detection of specific virus, virus isolation and identification, or anti virus antibody detection. Virus isolation and identification was more proper for virology study, molecular epidemiology study or for pathogenesis study than for diagnosis. The HI test even though this test was sensitive, but time consuming need more than 24 hours to performed. ELISA test was sensitive and specific for IgM and IgG identification, but need special place (serology laboratory) and reagent to performed. Rapid Diagnostic test based on IgM or IgG identification, the test was practiced no need special equipment or place to performed, with good sensitivity and specivicity. The recent advance for dengue diagnosis is NSI detection, the glyco protein produce by infected cells from day 1 to 9 after the onset of fever.</p><p>Keywords: Dengue Hemorrhagic Fever, diagnosis, antibody