Biostimulation of soil impacted by 45000 ppm waste motor oil and Phytoremediation with Zea mays by Burkholderia cepacia and Rhizobium elti

Soil contaminated with 45000 ppm of waste motor oil (WMO) is a relatively high concentration of a mixture of aliphatic and aromatic hydrocarbons (HC), inhibits mineralization of organic matter and its fertility. This WMO´ concentration is high according to mexican regulation: NOM-138-SEMARNAT/ SSA1-2012 (NOM-138) related to when it exceeds 4400 ppm/Kg of soil. The aims of the study were: i) BS of contaminated soil by 45000 ppm of WMO with vermicompost and bovine compost 3%, and ii) PR using Zea mays inoculated with B. cepacia and R. etli at value below the maximum allowable by NOM-138. The main response variable of the trial was initial and final concentration of WMO after BS. In PR by Z. mays and PGPB to reduce remain-ing WMO, were phenological response variables as: plant height and root length and biomass: aerial and root fresh and dry weight. Experimental data were analyzed by ANOVA and Tukey. Results showed that the BS of soil by 45000 ppm of WMO was reduced to 21000 ppm; subsequent FR sowing Z. mays inoculated by B. cepacia decreased to 1822.5 ppm, value below the maximum allowable by NOM-138. BS of contaminated soil by relatively high concentration of WMO and later FR sowing Z. mays and PGPR. Both are an alternative in its remediation that to apply each one alone