Identification anti-inflammatory effect of extract brown algae Sargassum sp. to mice (Mus musculus): A pilot study

Seaweed is one of natural resource beneath the sea has many benefits. Its contain has potential to used as medicine in dentistry, one of which is anti-inflammatory. Inflammation is response that appears when trauma, infection and tissue injury occurs. Sulphated polysaccharide in brown algae can be inhibit diseases associated with inflammation. The purpose of this study is to identify anti-inflammatory effect of extract brown seaweed Sargassum sp. to mice. This study used 18 male mices weighing 14-35g for age 2-4 months were obtained for one week before they use as animal experiment. Mices were divided into 3 groups (n=6). G1 : negative control and injected NaCMC solution 10 mg/35 g body weight. G2 : positive control and injected sodium diclofenac 0.1 mg/10 g body weight. G 3 : methanol extract of Sargassum sp. 6.175 mg/35 g body weight. After 30 minutes, 0.05 ml of 1% pepton injected to hind paw mice. The paw volume of mice at first hour until fourth hour after pepton injected was measured by plethysmometer. Analysis data using test of repeated ANOVA. The result showed there are decrease between volume of inflammation first hour until fourth hour in extract Sargassum sp. group. In conclusion, Sargassum sp. extract has effect anti-inflammatory