In the present study glucose oxidase (GOD) has been isolated from a culture filtrate of Penicillium notatum. The enzyme was purified by ammonium sulphate precipitation, diethylaminoethyl (DEAE) cellulose ion-exchange chromatography and Sephadex gel filtration. This protocol gave 16.47-fold purification and 25 % recovery of the enzyme. The optimum pH and temperature for the activity were 5.4 and 45 °C, respectively. The Km and vmax values for the enzyme were 10.5 mM and 456 U/mg, respectively. A detailed kinetic study of thermal inactivation was carried out. Both enthalpy of activation (ΔH*) and entropy of activation (ΔS*) decreased at higher temperatures. Moreover, free energy of denaturation (ΔG*) increased at higher temperature, making th...
Pyruvate has been determined at 16, 28 and 40 hours in a culture of Pseudomonas aeruginosa when grow...
Invertase is a commercially important enzyme used for hydrolysis of sucrose.The hydrolysis of sucros...
The thermal stability of enzyme-based biosensors is crucial in economic feasibility. In this study, ...
In the present study glucose oxidase (GOD) has been isolated from a culture filtrate of Penicillium ...
The study included the extraction and purification of glucose oxidase enzyme from Penicillium chryso...
Glucose oxidase (GOD, EC.1.1.3.4) specifically catalyzes the reaction of β-d-glucose to gluconic aci...
This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and...
A short method of purification of P.amagasakiense glucose oxidase has been achieved. Attempts to pre...
Glucose oxidase was an enzyme which catalyzed β-D-Glucose to gluconic acid and hydrogen peroxide. Gl...
A GH3 beta-glucosidase (BGL) from Penicillium brasilianum was purified to homogeneity after cultivat...
A GH3 β-glucosidase (BGL) from Penicillum brasilianum was purified to homogeneity after cultivation ...
Background: Amylases are used in various industrial processes and a key requirement for the efficien...
Glucose Oxidase (GOD), is a common flavoprotein from Aspergillus niger ATCC 9029 with a broad applic...
The aim of this study was kinetic analysis of the multi-enzyme solution produced from waste bread vi...
Glucose oxidase (GOx) is a flavoenzyme having applications in food and medical industries. However, ...
Pyruvate has been determined at 16, 28 and 40 hours in a culture of Pseudomonas aeruginosa when grow...
Invertase is a commercially important enzyme used for hydrolysis of sucrose.The hydrolysis of sucros...
The thermal stability of enzyme-based biosensors is crucial in economic feasibility. In this study, ...
In the present study glucose oxidase (GOD) has been isolated from a culture filtrate of Penicillium ...
The study included the extraction and purification of glucose oxidase enzyme from Penicillium chryso...
Glucose oxidase (GOD, EC.1.1.3.4) specifically catalyzes the reaction of β-d-glucose to gluconic aci...
This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and...
A short method of purification of P.amagasakiense glucose oxidase has been achieved. Attempts to pre...
Glucose oxidase was an enzyme which catalyzed β-D-Glucose to gluconic acid and hydrogen peroxide. Gl...
A GH3 beta-glucosidase (BGL) from Penicillium brasilianum was purified to homogeneity after cultivat...
A GH3 β-glucosidase (BGL) from Penicillum brasilianum was purified to homogeneity after cultivation ...
Background: Amylases are used in various industrial processes and a key requirement for the efficien...
Glucose Oxidase (GOD), is a common flavoprotein from Aspergillus niger ATCC 9029 with a broad applic...
The aim of this study was kinetic analysis of the multi-enzyme solution produced from waste bread vi...
Glucose oxidase (GOx) is a flavoenzyme having applications in food and medical industries. However, ...
Pyruvate has been determined at 16, 28 and 40 hours in a culture of Pseudomonas aeruginosa when grow...
Invertase is a commercially important enzyme used for hydrolysis of sucrose.The hydrolysis of sucros...
The thermal stability of enzyme-based biosensors is crucial in economic feasibility. In this study, ...