Partial quantification of pigments extracted from the zooxanthellate octocoral Sinularia flexibilis at varying irradiances

Chlorophyll-a (chl-a) and carotenoid pigments of the zooxanthellate octocoral Sinularia flexibilis were analyzed using high performance liquid chromatography following exposure to three light intensities for over 30 days. From the coral fragments located at different light intensities, a total carotenoid of >41 µg g-1 dry weight, including peridinin, xanthophylls (likely diadinoxanthin + diatoxanthin), and chl-a as the most abundant pigments, with minor contents of astaxantin and ß-carotene were detected. The whole content of chl-a weighed 5 µg g-1 dry weight in all coral colonies. Chl-a and carotenoids contributed 11.2% and 88.2%, respectively, to all pigments detected, and together accounted for 99.4% of the total pigments present. The highest contents of carotenoids and chl-a was observed in the coral grafts placed in an irradiance of 100 µmol quanta m-2 s-1; they showed lower ratios of total carotenoids: chl-a compared to those exposed to 400 µmol quanta m-2 s-1 after >30 days of incubation. The ratios of peridinin and xanthophylls with respect to chl-a from the colonies at 400 µmol quanta m-2 s-1 were approximately double those observed at irradiances of 100 and 200 µmol quanta m-2 s-1. Partial quantification of pigments in this study showed that the carotenoids of S. flexibilis showed a decrease at irradiances above 100 µmol quanta m-2 s-1, with the exception of an increase in ß-carotene at 200 µmol quanta m-2 s-1