Mechanisms regulating follicle wave patterns in the bovine estrous cycle investigated with a mathematical model

A normal bovine estrous cycle contains 2 or 3 waves of follicle development, and ovulation takes place in the last wave. However, the biological mechanisms that determine whether a cycle has 2 or 3 waves have not been elucidated. In a previous paper, we described a mathematical model of the bovine estrous cycle that generates cyclical fluctuations of hormones, follicles, and corpora lutea in estrous cycles of approximately 21 d for cows with a normal estrous cycle. The parameters in the model represent kinetic properties of the system with regard to synthesis, release, and clearance of hormones and growth and regression of follicles and corpora lutea. The initial model parameterization resulted in estrous cycles with 3 waves of follicular growth. Here, we use this model to explore which physiological mechanisms could affect the number of follicular waves. We hypothesized that some of the parameters related to follicle growth rate or to the time point of corpus luteum regression are likely candidates to affect the number of waves per cycle. We performed simulations with the model in which we varied the values of these parameters. We showed that variation of (combinations of) model parameters regulating follicle growth rate or time point of corpus luteum regression can change the model output from 3 to 2 waves of follicular growth in a cycle. In addition, alternating 2- and 3-wave cycles occurred. Some of the parameter changes seem to represent plausible biological mechanisms that could explain these follicular wave patterns. In conclusion, our simulations indicated likely parameters involved in the mechanisms that regulate the follicular wave pattern, and could thereby help to find causes of declined fertility in dairy cows