Micropropagation of Lisianthus (Eustoma grandiflorum cv. White) at In Vitro Conditions

Lisianthus (Eustoma grandiflorum cv. White) is an ornamental plant which has suitable marketing due to a variety of flower colours. The aim of the present study was to evaluate the effect of plant growth regulators on propagation and rooting of this plant. Axillary bud (node) was used as explants and MS medium supplemented with 0, 0.1, 0.2 and 2 mg/L of NAA and BA was applied. The results showed that the greatest shoot length (2.07 cm/plant) was obtained on a medium supplemented with 0.1 mg/L BA (without NAA). Maximum shoot number (5.80/plant) was produced in a medium containing 0.1 mg/L BA along with 0.2 mg/L NAA. Bud explants in culture media containing 0.2 mg/L NAA (without BA) and 0.1 mg/L NAA along with 2 mg/L BA produced the maximum node number (3.20/plant). The largest number of root (14.53/plant) and root length (3.87 cm/plant) were produced in media containing 0.2 mg/L NAA (without BA), also 0.2 mg/L BA plus 0.2 mg/L NAA and 0.2 mg/L BA without NAA, respectively. Explants produced flower on medium containing 0.1 mg/L BA along with 0.1 mg/L NAA at callus formation stage. Also, flower was produced from callus in medium containing 0.1 mg/L BA along with 2 mg/L NAA. Nearly 48% of the plantlets were able to survive on a medium containing a 1:1 ratio of peat and perlite