Fast and nondestructive method for leaf level chlorophyll estimation using hyperspectral LiDAR

We propose an empirical method for nondestructive estimation of chlorophyll in tree canopies. The first prototype of a full waveform hyperspectral LiDAR instrument has been developed by the Finnish Geodetic Institute (FGI). The instrument efficiently combines the benefits of passive and active remote sensing sensors. It is able to produce 3D point clouds with spectral information included for every point, which offers great potential in the field of environmental remote sensing. The investigation was conducted by using chlorophyll sensitive vegetation indices applied to hyperspectral LiDAR data and testing their performance in chlorophyll estimation. The amount of chlorophyll in vegetation is an important indicator of photosynthetic capacity and stress, and thus important for monitoring of forest condition and carbon sequestration on Earth. Performance of chlorophyll estimation was evaluated for 27 published vegetation indices applied to waveform LiDAR collected from ten Scots pine shoots. Reference data were collected by laboratory chlorophyll concentration analysis. The performance of the indices in chlorophyll estimation was determined by linear regression and leave-one-out cross-validation. The chlorophyll estimates derived from hyperspectral LiDAR linearly correlate with the laboratory analyzed chlorophyll concentrations, and they are able to represent a range of chlorophyll concentrations in Scots pine shoots (R2 = 0.88, RMSE = 0.10 mg/g). Furthermore, they are insensitive to measurement scale as nearly the same values of vegetation indices were measured in natural setting while scanning the whole canopy and from clipped shoots re-measured with hyperspectral LiDAR in laboratory. The results indicate that the hyperspectral LiDAR instrument has the potential to estimate vegetation biochemical parameters such as the chlorophyll concentration. The instrument holds much potential in various environmental applications and provides a significant improvement over single wavelength LiDAR or passive optical systems for environmental remote sensing