A modified thermal asymmetric interlaced polymerase chain reaction was performed to obtain the first yeast laccase gene (YlLac) from the isolated yeast Yarrowia lipolytica. The 1557-bp full-length cDNA of YlLac encoded a mature laccase protein containing 519 amino acids preceded by a signal peptide of 19 amino acids, and the YlLac gene was expressed in the yeast Pichia pastoris. YlLac is a monomeric glycoprotein with a molecular mass of ~55 kDa as determined by polyacrylamide-gel electrophoresis. It showed a higher catalytic efficiency towards 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (kcat/Km = 17.5 s(-1) μM(-1)) and 2,6-dimethoxyphenol (kcat/Km = 16.1 s(-1) μM(-1)) than other reported laccases. The standard redox potential of the ...
Laccase from Myceliophthora thermophila (MtL) was expressed in functional form in Saccharomyces cere...
International audienceThree laccases, a natural form and two recombinant forms obtained from two dif...
We obtained structural and functional characterization of a recombinant Laccase from Rigidoporus lig...
<div><p>A modified thermal asymmetric interlaced polymerase chain reaction was performed to obtain t...
Amodified thermal asymmetric interlaced polymerase chain reaction was performed to ob-tain the first...
An extracellular laccase-producing yeast was isolated from soil and identified as Yarrowia lipolytic...
The lcc1 gene coding for the laccase from Trametes versicolor DSM11269 was cloned into the genome of...
Numerous heterologous proteins have been successfully producedin Y. lipolytica, a dimorphic yeast wi...
International audiencePycnoporus cinnabarinus lac1 gene was expressed in Yarrowia lipolytica. Differ...
Laccases are used in numerous applications, from green degradation of various xenobiotic compounds, ...
Investigation of Bacterial Laccase Expression in The Yeast System The majority of chemical processes...
International audienceAims: Exploitation of natural biodiversity in species Pycnoporus coccineus and...
Laccases belong to the family of multicopper oxidases and they oxidise phenolic compounds with broad...
This work reports for the first time the secretory expression of the small laccase (SLAC) from Strep...
In this study, a novel laccase gene, named as Cplcc1, and its corresponding cDNA were isolated and c...
Laccase from Myceliophthora thermophila (MtL) was expressed in functional form in Saccharomyces cere...
International audienceThree laccases, a natural form and two recombinant forms obtained from two dif...
We obtained structural and functional characterization of a recombinant Laccase from Rigidoporus lig...
<div><p>A modified thermal asymmetric interlaced polymerase chain reaction was performed to obtain t...
Amodified thermal asymmetric interlaced polymerase chain reaction was performed to ob-tain the first...
An extracellular laccase-producing yeast was isolated from soil and identified as Yarrowia lipolytic...
The lcc1 gene coding for the laccase from Trametes versicolor DSM11269 was cloned into the genome of...
Numerous heterologous proteins have been successfully producedin Y. lipolytica, a dimorphic yeast wi...
International audiencePycnoporus cinnabarinus lac1 gene was expressed in Yarrowia lipolytica. Differ...
Laccases are used in numerous applications, from green degradation of various xenobiotic compounds, ...
Investigation of Bacterial Laccase Expression in The Yeast System The majority of chemical processes...
International audienceAims: Exploitation of natural biodiversity in species Pycnoporus coccineus and...
Laccases belong to the family of multicopper oxidases and they oxidise phenolic compounds with broad...
This work reports for the first time the secretory expression of the small laccase (SLAC) from Strep...
In this study, a novel laccase gene, named as Cplcc1, and its corresponding cDNA were isolated and c...
Laccase from Myceliophthora thermophila (MtL) was expressed in functional form in Saccharomyces cere...
International audienceThree laccases, a natural form and two recombinant forms obtained from two dif...
We obtained structural and functional characterization of a recombinant Laccase from Rigidoporus lig...