Gene profiling in atherosclerosis reveals a key role for small inducible cytokines: validation using a novel monocyte chemoattractant protein monoclonal antibody
- Lutgens, E.
- Faber, B.C.G.
- Schapira, K.B.
- Evelo, C.T.
- van Haaften, R.
- Heeneman, S.
- Cleutjens, K.B.J.M.
- Bijnens, A.P.J.J.
- Beckers, L.
- Porter, J.G.
- Mackay, C.R.
- Rennert, P.
- Bailly, V.
- Jarpe, M.
- Dolinski, B.
- Koteliansky, V.
- de Fougerolles, T.
- Daemen, M.J.
DOIAbstract
BACKGROUND: Pathological aspects of atherosclerosis are well described, but gene profiles during atherosclerotic plaque progression are largely unidentified. METHODS AND RESULTS: Microarray analysis was performed on mRNA of aortic arches of ApoE-/- mice fed normal chow (NC group) or Western-type diet (WD group) for 3, 4.5, and 6 months. Of 10 176 reporters, 387 were differentially (>2x) expressed in at least 1 group compared with a common reference (ApoE-/-, 3- month NC group). The number of differentially expressed genes increased during plaque progression. Time-related expression clustering and functional grouping of differentially expressed genes suggested important functions for genes involved in inflammation (especially the small induc...
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