BACKGROUND: Genetic modifications can improve the therapeutic efficacy of mesenchymal stem cell (MSC) transplantation in myocardial infarction. However, so far, the efficiency of MSC modification is very low. Seeking for a more efficient way of MSC modification, we investigated the possibility of employing the intercellular trafficking capacity of the herpes simplex virus type-1 tegument protein VP22 on the enhancement of MSC modification. METHODS: Plasmids pVP22-myc, pVP22-EGFP, pEGFP-VP22, pVP22-hBcl-xL and phBcl-xL-VP22 were constructed for the expressions of the myc-tagged VP22 and the fusion proteins VP22-EGFP, EGFP-VP22, VP22-hBcl-xL and hBcl-xL-VP22. MSCs were isolated from rat bone marrow and the surface markers were identified by F...
BACKGROUND Mesenchymal stem cells (MSCs) are multipotent cells residing in the connective tissue of ...
BACKGROUND: Mesenchymal stem cells (MSCs) are multipotent cells residing in the connective tissue of...
We examine biochemical characteristics of the herpes simplex virus (HSV) tegument protein VP22 by ge...
Genetic modifications can improve the therapeutic efficacy of mesenchymal stem cell (MSC) transplant...
The type I herpes simplex virus VP22 tegument protein is abundant and well known for its ability to ...
Background: The intercellular transport properties of the herpes simplex virus (HSV) protein VP22 ha...
International audienceVP22, a structural protein from herpes simplex virus type I, exhibits the uniq...
AbstractWe show that the HSV-1 structural protein VP22 has the remarkable property of intercellular ...
Recently, a new approach for direct protein transfer to mammalian cells based on the herpes simplex ...
Background: The inefficiency of herpes simplex virus thymidine kinase (TK) gene transfer and toxicit...
<p>COS-1 cells transfected with 0.5 µg pVP22-EGFP (A–D and I–L) and pEGFP-VP22 (E–H and M–P) DNA wer...
<p>COS-1 cells transfected with 0.5 µg pVP22-myc DNA were trypsinized 24 hours after transfection an...
Recently, a new approach for direct protein transfer to mammalian cells based on the herpes simplex ...
We examine biochemical characteristics of the herpes simplex virus (HSV) tegument protein VP22 by ge...
Suicide gene therapy is a promising approach for the treatment of cancer. Current protocols, however...
BACKGROUND Mesenchymal stem cells (MSCs) are multipotent cells residing in the connective tissue of ...
BACKGROUND: Mesenchymal stem cells (MSCs) are multipotent cells residing in the connective tissue of...
We examine biochemical characteristics of the herpes simplex virus (HSV) tegument protein VP22 by ge...
Genetic modifications can improve the therapeutic efficacy of mesenchymal stem cell (MSC) transplant...
The type I herpes simplex virus VP22 tegument protein is abundant and well known for its ability to ...
Background: The intercellular transport properties of the herpes simplex virus (HSV) protein VP22 ha...
International audienceVP22, a structural protein from herpes simplex virus type I, exhibits the uniq...
AbstractWe show that the HSV-1 structural protein VP22 has the remarkable property of intercellular ...
Recently, a new approach for direct protein transfer to mammalian cells based on the herpes simplex ...
Background: The inefficiency of herpes simplex virus thymidine kinase (TK) gene transfer and toxicit...
<p>COS-1 cells transfected with 0.5 µg pVP22-EGFP (A–D and I–L) and pEGFP-VP22 (E–H and M–P) DNA wer...
<p>COS-1 cells transfected with 0.5 µg pVP22-myc DNA were trypsinized 24 hours after transfection an...
Recently, a new approach for direct protein transfer to mammalian cells based on the herpes simplex ...
We examine biochemical characteristics of the herpes simplex virus (HSV) tegument protein VP22 by ge...
Suicide gene therapy is a promising approach for the treatment of cancer. Current protocols, however...
BACKGROUND Mesenchymal stem cells (MSCs) are multipotent cells residing in the connective tissue of ...
BACKGROUND: Mesenchymal stem cells (MSCs) are multipotent cells residing in the connective tissue of...
We examine biochemical characteristics of the herpes simplex virus (HSV) tegument protein VP22 by ge...