Radioimmunoassay for pancreatic glucagon based on specific derivatisation of the hormone

A specific glucagon antiserum was generated in rabbits using a derivatisation of the methionine residue in glucagon. A sensitive and specific assay for glucagon has been developed. Interference with the assay system by plasma factors was abolished by both extraction of plasma samples and the production of glucagon-free plasma for each individual. Chemicals/CAS: glucagon, 11140-85-5, 62340-29-8, 9007-92-5; Aprotinin, 9087-70-1; Arginine, 74-79-3; Glucagon, 9007-92-5; S-methylglucagon, 68600-12-