A method for the transformation of hybridoma cell lines with improved efficiency : its use in the production of bispecific monoclonal antibodies

A method was investigated to transduce a specific drug resistance marker, coded on a bacterial plasmid construct, to a hybridoma cell line using electroporation. We tested the influence of several buffers and the form of the DNA on cell viability and stable transfection frequency. We found a tenfold higher stable transfection frequency using DMEM + FCS as eiectroporation buffer, as compared with HBS. Furthermore, using DMEM + FCS, plasmid linearisation had little effect on the stable transfection frequency. This method proved to be succesful when isolating quadromas for the production of bispecific antibodies