In the present study, a novel pre-miniproinsulin analogue was designed to have a short 9 residue sequence replacing the 35 residue C-chain, one lysine and one arginine added to the C-terminus of the B-chain in combination with glycine and arginine substitution at A21 and B29, respectively, and a 16-residue fusion partner comprising the pentapeptide sequence (PSDKP) of the N-terminus of human tumor necrosis factor-α (TNF-α), 6 histidine residues for Ni2+ chelated affinity purification and a pentapeptide ending with methionine for ease of chemical cleavage fused at the N-terminus. Homology modeling of the designed protein against miniproinsulin (protein databank file 1 efeA) as a template showed that the distance between the α-carbons of the ...
Background: Human insulin-like growth factor type 1 (hIGF-1) is a protein consisting of 70 amino aci...
Background: Human insulin-like growth factor type 1 (hIGF-1) is a protein consisting of 70 amino aci...
The increased incidence of diabetes, coupled with the introduction of alternative insulin delivery m...
AbstractIn the present study, a novel pre-miniproinsulin analogue was designed to have a short 9 res...
Synthesis of human mini-proinsulin precursors was investigated in controlled fed-batch cultures at h...
Background: Recombinant insulin glargine, a long-acting analog of insulin, is expressed as proinsuli...
In order to prepare a novel human insulin analogue substituted with homoserine at B30 position, (B30...
A 13 amino acid sequence, CRVARGDWNDNYC, originated from disintegrin eristostatin, was introduced in...
The uncontrolled incorporation of non-canonical branched chain amino acids (ncBCAAs) such as norleuc...
Non-canonical proline residues expand the chemical space about proline, while maintaining some confo...
The construction of a gene encoding Lys-human proinsulin, its direct expression in E. coli, and the ...
More effective production of human insulin is important, because insulin is the main medication that...
A fusion gene encoding double-C-peptide human proinsulin was constructed by insertion of a DNA fragm...
abstract: The C6T single-chain variable fragment (scFv) is an antibody fragment designed as a potent...
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant ...
Background: Human insulin-like growth factor type 1 (hIGF-1) is a protein consisting of 70 amino aci...
Background: Human insulin-like growth factor type 1 (hIGF-1) is a protein consisting of 70 amino aci...
The increased incidence of diabetes, coupled with the introduction of alternative insulin delivery m...
AbstractIn the present study, a novel pre-miniproinsulin analogue was designed to have a short 9 res...
Synthesis of human mini-proinsulin precursors was investigated in controlled fed-batch cultures at h...
Background: Recombinant insulin glargine, a long-acting analog of insulin, is expressed as proinsuli...
In order to prepare a novel human insulin analogue substituted with homoserine at B30 position, (B30...
A 13 amino acid sequence, CRVARGDWNDNYC, originated from disintegrin eristostatin, was introduced in...
The uncontrolled incorporation of non-canonical branched chain amino acids (ncBCAAs) such as norleuc...
Non-canonical proline residues expand the chemical space about proline, while maintaining some confo...
The construction of a gene encoding Lys-human proinsulin, its direct expression in E. coli, and the ...
More effective production of human insulin is important, because insulin is the main medication that...
A fusion gene encoding double-C-peptide human proinsulin was constructed by insertion of a DNA fragm...
abstract: The C6T single-chain variable fragment (scFv) is an antibody fragment designed as a potent...
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant ...
Background: Human insulin-like growth factor type 1 (hIGF-1) is a protein consisting of 70 amino aci...
Background: Human insulin-like growth factor type 1 (hIGF-1) is a protein consisting of 70 amino aci...
The increased incidence of diabetes, coupled with the introduction of alternative insulin delivery m...