A Modified Carbon Monoxide Breath Test for Measuring Erythrocyte Lifespan in Small Animals

Yong-Jian Ma
Hou-De Zhang
Yong-Qiang Ji
Guo-Liang Zhu
Jia-Liang Huang
Li-Tao Du
Ping Cao
De-Yue Zang
Ji-Hui Du
Rong Li
Lei Wang

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Publication date

January 2016

DOI

10.1155/2016/7173156

Publisher

Hindawi Limited

ISSN

2314-6133

Journal

2314-6141

Abstract

This study was to develop a CO breath test for RBC lifespan estimation of small animals. The ribavirin induced hemolysis rabbit models were placed individually in a closed rebreath cage and air samples were collected for measurement of CO concentration. RBC lifespan was calculated from accumulated CO, blood volume, and hemoglobin concentration data. RBC lifespan was determined in the same animals with the standard biotin-labeling method. RBC lifespan data obtained by the CO breath test method for control (CON, 49.0±5.9 d) rabbits, rabbits given 10 mg/kg·d−1 of ribavirin (RIB10, 31.0±4.0 d), and rabbits given 20 mg/kg·d−1 of ribavirin (RIB20, 25.0±2.9 d) were statistically similar (all p>0.05) to and linearly correlated (r=0.96, p<0.01) with...