The full-length dystrophin protein isoform of 427 kDa (Dp427), the absence of which represents the principal abnormality in X-linked muscular dystrophy, is difficult to identify and characterize by routine proteomic screening approaches of crude tissue extracts. This is probably related to its large molecular size, its close association with the sarcolemmal membrane, and its existence within a heterogeneous glycoprotein complex. Here, we used a careful extraction procedure to isolate the total protein repertoire from normal versus dystrophic mdx-4cv skeletal muscles, in conjunction with label-free mass spectrometry, and successfully identified Dp427 by proteomic means. In contrast to a considerable number of previous comparative studies of ...
The highly progressive neuromuscular disorder dystrophinopathy is triggered by primary abnormalities...
Abstract. Dystrophin, the protein product of the hu-man Duchenne muscular dystrophy gene, exists in ...
Duchenne muscular dystrophy is the most frequent neuromuscular disorder of childhood. Although this ...
The full-length dystrophin protein isoform of 427 kDa (Dp427), the absence of which represents the p...
In skeletal muscle, the dystrophin-glycoprotein complex forms a membrane-associated assembly of rela...
The almost complete loss of the membrane cytoskeletal protein dystrophin and concomitant drastic red...
Supramolecular membrane complexes of low abundance are difficult to study by routine bioanalytical t...
The primary deficiency in the membrane cytoskeletal protein dystrophin results in complex changes in...
Extraocular muscles (EOMs) represent a specialized type of contractile tissue with unique cellular, ...
Extraocular muscles (EOMs) represent a specialized type of contractile tissue with unique cellular, ...
AbstractThe development of advanced mass spectrometric methodology has decisively enhanced the analy...
The development of advanced mass spectrometric methodology has decisively enhanced the analytical ca...
Duchenne muscular dystrophy is a highly complex multi-system disorder caused by primary abnormalitie...
Although the membrane cytoskeletal protein dystrophin of 427kDa and its tightly associated glycoprot...
Duchenne Muscular Dystrophy is a lethal childhood disorder which results in progressive muscle weakn...
The highly progressive neuromuscular disorder dystrophinopathy is triggered by primary abnormalities...
Abstract. Dystrophin, the protein product of the hu-man Duchenne muscular dystrophy gene, exists in ...
Duchenne muscular dystrophy is the most frequent neuromuscular disorder of childhood. Although this ...
The full-length dystrophin protein isoform of 427 kDa (Dp427), the absence of which represents the p...
In skeletal muscle, the dystrophin-glycoprotein complex forms a membrane-associated assembly of rela...
The almost complete loss of the membrane cytoskeletal protein dystrophin and concomitant drastic red...
Supramolecular membrane complexes of low abundance are difficult to study by routine bioanalytical t...
The primary deficiency in the membrane cytoskeletal protein dystrophin results in complex changes in...
Extraocular muscles (EOMs) represent a specialized type of contractile tissue with unique cellular, ...
Extraocular muscles (EOMs) represent a specialized type of contractile tissue with unique cellular, ...
AbstractThe development of advanced mass spectrometric methodology has decisively enhanced the analy...
The development of advanced mass spectrometric methodology has decisively enhanced the analytical ca...
Duchenne muscular dystrophy is a highly complex multi-system disorder caused by primary abnormalitie...
Although the membrane cytoskeletal protein dystrophin of 427kDa and its tightly associated glycoprot...
Duchenne Muscular Dystrophy is a lethal childhood disorder which results in progressive muscle weakn...
The highly progressive neuromuscular disorder dystrophinopathy is triggered by primary abnormalities...
Abstract. Dystrophin, the protein product of the hu-man Duchenne muscular dystrophy gene, exists in ...
Duchenne muscular dystrophy is the most frequent neuromuscular disorder of childhood. Although this ...