Apoptosis Induction in Human Leukemia Cell Lines by Gold Nanoparticles Synthesized Using the Green Biosynthetic Approach
- Farideh Namvar
- Heshu Sulaiman Rahman
- Rosfarizan Mohamad
- Abdullah Rasedee
- Swee Keong Yeap
- Max Stanley Chartrand
- Susan Azizi
- Paridah Mohd Tahir
DOIAbstract
Gold nanoparticles were grown on Sargassum muticum water extract (S-GNPs) using the green biosynthetic approach. The nanoparticles were characterized using UV-visible spectroscopy, zeta potential, and transmission electron microscopy (TEM). The resulting S-GNPs were spherical and crystalline with a size of <10 nm. The in vitro anticancer activity was demonstrated in human leukemia cell lines. The cancer cells were treated with different concentrations of S-GNPs, and calorimetric (MTT) assay used for the cytotoxicity test, which resulted in an IC50 value of 4.22 ± 1.12, 5.71 ± 1.4, 6.55 ± 0.9, and 7.29 ± 1.7 μg/mL for each of the K562, HL-60, Jurkat, and CEM-ss cells, respectively. Thus, the K562 was selected for the next experiments. Furth...
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