Unifying candidate gene and GWAS Approaches in Asthma.

Sven Michel
Liming Liang
Martin Depner
Norman Klopp
Andreas Ruether
Ashish Kumar
Michaela Schedel
Christian Vogelberg
Erika von Mutius
Andrea von Berg
Albrecht Bufe
Ernst Rietschel
Andrea Heinzmann
Otto Laub
Burkhard Simma
Thomas Frischer
Jon Genuneit
Ivo G Gut
Stefan Schreiber
Mark Lathrop
Thomas Illig
Michael Kabesch

Open link

DOI

10.1371/journal.pone.0013894

Publisher

Public Library of Science (PLoS)

Journal

issn:1932-6203

Abstract

The first genome wide association study (GWAS) for childhood asthma identified a novel major susceptibility locus on chromosome 17q21 harboring the ORMDL3 gene, but the role of previous asthma candidate genes was not specifically analyzed in this GWAS. We systematically identified 89 SNPs in 14 candidate genes previously associated with asthma in >3 independent study populations. We re-genotyped 39 SNPs in these genes not covered by GWAS performed in 703 asthmatics and 658 reference children. Genotyping data were compared to imputation data derived from Illumina HumanHap300 chip genotyping. Results were combined to analyze 566 SNPs covering all 14 candidate gene loci. Genotyped polymorphisms in ADAM33, GSTP1 and VDR showed effects with p-va...