Implications of targeted genomic disruption of β-catenin in BxPC-3 pancreatic adenocarcinoma cells.
- Petter Angell Olsen
- Nina Therese Solberg
- Kaja Lund
- Tore Vehus
- Monika Gelazauskaite
- Steven Ray Wilson
- Stefan Krauss
DOIAbstract
Pancreatic adenocarcinoma (PA) is among the most aggressive human tumors with an overall 5-year survival rate of <5% and available treatments are only minimal effective. WNT/β-catenin signaling has been identified as one of 12 core signaling pathways that are commonly mutated in PA. To obtain more insight into the role of WNT/β-catenin signaling in PA we established human PA cell lines that are deficient of the central canonical WNT signaling protein β-catenin by using zinc-finger nuclease (ZFN) mediated targeted genomic disruption in the β-catenin gene (CTNNB1). Five individual CTNNB1 gene disrupted clones (BxPC3ΔCTNNB1) were established from a BxPC-3 founder cell line. Despite the complete absence of β-catenin, all clones displayed normal...
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