Identification of cellular calcium binding protein calmodulin as a regulator of rotavirus A infection during comparative proteomic study.
- Shiladitya Chattopadhyay
- Trayambak Basak
- Mukti Kant Nayak
- Gourav Bhardwaj
- Anupam Mukherjee
- Rahul Bhowmick
- Shantanu Sengupta
- Oishee Chakrabarti
- Nabendu S Chatterjee
- Mamta Chawla-Sarkar
DOIAbstract
Rotavirus (RV) being the major diarrhoegenic virus causes around 527000 children death (<5 years age) worldwide. In cellular environment, viruses constantly adapt and modulate to survive and replicate while the host cell also responds to combat the situation and this results in the differential regulation of cellular proteins. To identify the virus induced differential expression of proteins, 2D-DIGE (Two-dimensional Difference Gel Electrophoresis) based proteomics was used. For this, HT-29 cells were infected with RV strain SA11 for 0 hours, 3 hours and 9 hours post infection (hpi), differentially expressed spots were excised from the gel and identified using MALDI-TOF/TOF mass spectrometry. 2D-DIGE based proteomics study identified 32 dif...
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