Nucleotide excision repair is not induced in human embryonic lung fibroblasts treated with environmental pollutants.
- Pavel Rossner
- Andrea Mrhalkova
- Katerina Uhlirova
- Milada Spatova
- Andrea Rossnerova
- Helena Libalova
- Jana Schmuczerova
- Alena Milcova
- Jan Topinka
- Radim J Sram
DOIAbstract
The cellular response to genotoxic treatment depends on the cell line used. Although tumor cell lines are widely used for genotoxicity tests, the interpretation of the results may be potentially hampered by changes in cellular processes caused by malignant transformation. In our study we used normal human embryonic lung fibroblasts (HEL12469 cells) and tested their response to treatment with benzo[a]pyrene (B[a]P) and extractable organic matter (EOM) from ambient air particles <2.5 µm (PM2.5) collected in two Czech cities differing in levels and sources of air pollution. We analyzed multiple endpoints associated with exposure to polycyclic aromatic hydrocarbons (PAHs) including the levels of bulky DNA adducts and the nucleotide excision rep...
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