Ribonucleotide reductase (RNR) enzyme is composed of the homodimeric RRM1 and RRM2 subunits, which together form a heterotetramic active enzyme that catalyzes the de novo reduction of ribonucleotides to generate deoxyribonucleotides (dNTPs), which are required for DNA replication and DNA repair processes. In this study, we show that ablation of RRM1 and RRM2 by siRNA induces G1/S phase arrest, phosphorylation of Chk1 on Ser345 and phosphorylation of γ-H2AX on S139. Combinatorial ablation of RRM1 or RRM2 and Chk1 causes a dramatic accumulation of γ-H2AX, a marker of double-strand DNA breaks, suggesting that activation of Chk1 in this context is essential for suppression of DNA damage. Significantly, we demonstrate for the first time that Chk...
The pathway determining malignant cellular transformation, which depends upon mutation of the BRCA1 ...
<p>Cells were transfected with Chk1, RRM1, RRM2, RRM1/Chk1, RRM2/Chk1, and Luciferase control (untre...
Cells exposed to hypoxia, experience replication stress but do not accumulate DNA damage, suggesting...
<div><p>Ribonucleotide reductase (RNR) enzyme is composed of the homodimeric RRM1 and RRM2 subunits,...
The ribonucleotide reductase (RNR) complex, composed of a catalytic subunit (RRM1) and a regulatory ...
<p>(a) At 30 h after siRNA transfections of the luciferase, RRM1, and RRM2 siRNA duplexes, extracts ...
F-box proteins are the substrate binding subunits of SCF (Skp1-Cul1-F-box protein) ubiquitin ligase...
F-box proteins are the substrate binding subunits of SCF (Skp1-Cul1-F-box protein) ubiquitin ligase ...
SummaryF-box proteins are the substrate binding subunits of SCF (Skp1-Cul1-F-box protein) ubiquitin ...
Sufficient pools of deoxyribonucleotide triphophates (dNTPs) are essential for the high fidelity rep...
F-box proteins are the substrate binding subunits of SCF (Skp1-Cul1-F-box protein) ubiquitin ligase ...
Nucleotide levels play a critical role in genome stability, and proper regulation of these pools is ...
Mammalian cells can choose either nonhomologous end joining (NHEJ) or homologous recombination (HR) ...
Rad53 protein, the yeast orthologue of the human checkpoint kinase Chk2, presents two highly conserv...
SummarySynthesis of deoxynucleoside triphosphates (dNTPs) is required for both DNA replication and D...
The pathway determining malignant cellular transformation, which depends upon mutation of the BRCA1 ...
<p>Cells were transfected with Chk1, RRM1, RRM2, RRM1/Chk1, RRM2/Chk1, and Luciferase control (untre...
Cells exposed to hypoxia, experience replication stress but do not accumulate DNA damage, suggesting...
<div><p>Ribonucleotide reductase (RNR) enzyme is composed of the homodimeric RRM1 and RRM2 subunits,...
The ribonucleotide reductase (RNR) complex, composed of a catalytic subunit (RRM1) and a regulatory ...
<p>(a) At 30 h after siRNA transfections of the luciferase, RRM1, and RRM2 siRNA duplexes, extracts ...
F-box proteins are the substrate binding subunits of SCF (Skp1-Cul1-F-box protein) ubiquitin ligase...
F-box proteins are the substrate binding subunits of SCF (Skp1-Cul1-F-box protein) ubiquitin ligase ...
SummaryF-box proteins are the substrate binding subunits of SCF (Skp1-Cul1-F-box protein) ubiquitin ...
Sufficient pools of deoxyribonucleotide triphophates (dNTPs) are essential for the high fidelity rep...
F-box proteins are the substrate binding subunits of SCF (Skp1-Cul1-F-box protein) ubiquitin ligase ...
Nucleotide levels play a critical role in genome stability, and proper regulation of these pools is ...
Mammalian cells can choose either nonhomologous end joining (NHEJ) or homologous recombination (HR) ...
Rad53 protein, the yeast orthologue of the human checkpoint kinase Chk2, presents two highly conserv...
SummarySynthesis of deoxynucleoside triphosphates (dNTPs) is required for both DNA replication and D...
The pathway determining malignant cellular transformation, which depends upon mutation of the BRCA1 ...
<p>Cells were transfected with Chk1, RRM1, RRM2, RRM1/Chk1, RRM2/Chk1, and Luciferase control (untre...
Cells exposed to hypoxia, experience replication stress but do not accumulate DNA damage, suggesting...