Assessing the intralobular ducts in mouse mandibular gland

In order to quantify the types of intralobular ducts, we used five adult male mice, sacrificed by cervical dislocation, after exposure to anesthetic. After euthanasing the animals, the mandibular glands were histologically processed. We sectioned the tissue at 5 µm thickness and stained the slides using Goldner’s trichrome staining procedure. We captured images on four different microscopic fields for each animal, subsequently counting each type of intralobulary duct. The granular ducts were the most numerous, followed by the intermediary ones and striated ducts with the lowest number