Fast analysis of potassium, sodium, calcium, and magnesium cations in total parenteral nutrition formulations with the Wyn-CE Capillary Electrophoresis System coupled with a contactless conductivity detection

A simple and fast CE method developed on the new modular Wyn-CE system coupled with a sensitive contactless conductivity detection was described for the analysis of potassium, sodium, calcium, and magnesium cations in Total Parenteral Nutrition (TPN) formulations. The used background electrolyte is a mixture of 50 mM acetic acid and 12 mM L-Histidine, pH 4.1. With this electrolyte, the separation of the targeted cations was achieved in 2 min. Obtained limits of detection were comprised between 1 and 1.5 μM. The developed method was then validated and trueness values between 98.5% and 102.3% were obtained with repeatability values of 0.9-1.3%. This validated method was finally successfully applied to TPN quality control