Evaluation of Different Culture Media for Improvement in Bioinsecticides Production by Indigenous Bacillus thuringiensis and Their Application against Larvae of Aedes aegypti
- Patil Chandrashekhar Devidas
- Borase Hemant Pandit
- Patil Satish Vitthalrao
DOIAbstract
Production of indigenous isolate Bacillus thuringiensis sv2 (Bt sv2) was checked on conventional and nonconventional carbon and nitrogen sources in shake flasks. The effects on the production of biomass, toxin production, and spore formation capability of mosquito toxic strain were determined. Toxicity differs within the same strain depending on the growth medium. Bt sv2 produced with pigeon pea and soya bean flour were found highly effective with LC50<4 ppm against larvae of Aedes aegypti. These results were comparable with bacteria produced from Luria broth as a reference medium. Cost-effective analyses have revealed that production of biopesticide from test media is highly economical. The cost of production of Bt sv2 with soya bean flour...
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