Transcription activator-like effector nucleases (TALENs) enable genome engineering in cell culture and many organisms. Recently, the GoldyTALEN scaffold was shown to readily introduce mutations in zebrafish (Danio rerio) and livestock through non-homologous end joining (NHEJ) and homology-directed repair (HDR). To deploy the GoldyTALEN system for high-throughput mutagenesis in model organisms, a simple design with high efficacy is desirable. We tested the in vivo efficacy of a simplified 15-RVD GoldyTALEN design (spacer between 13-20 bp and T nucleotide preceding each TALEN binding site) in zebrafish. All 14 tested TALEN pairs (100%) introduced small insertions and deletions at somatic efficacy ranging from 24 to 86%, and mutations were inh...
Recently, it has been shown that targeted mutagenesis using zinc-finger nucleases (ZFNs) and transcr...
To the Editor: Miller et al. recently described a TALE nuclease architecture for performing efficien...
Customized zinc finger nucleases (ZFNs) form the basis of a broadly applicable tool for highly effic...
Transcription activator-like effector nucleases (TALENs) enable genome engineering in cell culture a...
The zebrafish (Danio rerio) is increasingly being used to study basic vertebrate biology and human d...
Transcription activator-like effector nucleases (TALENs) are powerful new research tools that enable...
Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-L...
Transcription activator like effector nucleases (TALENs) is a promising approach to disrupt intended...
Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-L...
<div><p>Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Act...
Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-L...
Transcription activator-like effector nucleases (TALENs) are engineered endonucleases composed of a ...
Zebrafish is a valuable model organism to study vertebrate development, organ regeneration and to ge...
<div><p>Recently, it has been shown that targeted mutagenesis using zinc-finger nucleases (ZFNs) and...
<div><p>The zebrafish is a powerful experimental system for uncovering gene function in vertebrate o...
Recently, it has been shown that targeted mutagenesis using zinc-finger nucleases (ZFNs) and transcr...
To the Editor: Miller et al. recently described a TALE nuclease architecture for performing efficien...
Customized zinc finger nucleases (ZFNs) form the basis of a broadly applicable tool for highly effic...
Transcription activator-like effector nucleases (TALENs) enable genome engineering in cell culture a...
The zebrafish (Danio rerio) is increasingly being used to study basic vertebrate biology and human d...
Transcription activator-like effector nucleases (TALENs) are powerful new research tools that enable...
Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-L...
Transcription activator like effector nucleases (TALENs) is a promising approach to disrupt intended...
Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-L...
<div><p>Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Act...
Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-L...
Transcription activator-like effector nucleases (TALENs) are engineered endonucleases composed of a ...
Zebrafish is a valuable model organism to study vertebrate development, organ regeneration and to ge...
<div><p>Recently, it has been shown that targeted mutagenesis using zinc-finger nucleases (ZFNs) and...
<div><p>The zebrafish is a powerful experimental system for uncovering gene function in vertebrate o...
Recently, it has been shown that targeted mutagenesis using zinc-finger nucleases (ZFNs) and transcr...
To the Editor: Miller et al. recently described a TALE nuclease architecture for performing efficien...
Customized zinc finger nucleases (ZFNs) form the basis of a broadly applicable tool for highly effic...