STUDI DEGRADASI KINETIK KLOROFIL PADA DAUN (Garcinia Mangostana L dan Morinda Citrifolia L) MENGGUNAKAN METODE FLUORESENSI

STUDI DEGRADASI KINETIK KLOROFIL PADA DAUN (Garcinia Mangostana L dan Morinda Citrifolia L) MENGGUNAKAN METODE FLUORESENSI\ud Masni1), Dahlang Tahir1), Isnaeni2)\ud 1)Departemen Fisika, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Hasanuddin Jl. Perintis Kemerdekaan 10, Makassar, Sulawesi Selatan.\ud 2) Pusat Penelitian Fisika, Lembaga Ilmu Pengetahuan Indonesia, kawasan PUSPIPTEK Gedung 440-442, Tangerang Selatan, 15314\ud E-mail : dtahir@fmipa.unhas.ac.id, masnisineng@gmail.com\ud \ud ABSTRAK\ud Telah dilakukan penelitian tentang studi degradasi kinetik klorofil pada daun (Garcinia Mangostana L dan Morinda citrifolia L) menggunakan metode fluoresensi. Penelitian ini bertujuan untuk mendeteksi adanya klorofil dan menentukan waktu degradasi kinetik fluoresensi klorofil pada daun real menggunakan spektrometer HR2000. Metode ini menggunakan laser dengan fluoresensi 420 (nm). Sampel yang digunakan yaitu daun manggis dan daun mengkudu. Pengukuran ini dilakukan pada tiga bagian daun yaitu bagian pangkal, bagian tengah, dan bagian ujung daun. Dari hasil penelitian ini diperoleh bahwa emisi fluoresensi untuk spektrum klorofil daun manggis dan mengkudu pada setiap sampel berada pada panjang gelombang dari rentang 679-735 (nm), artinya bahwa klorofil menyerap warna merah. Waktu degradasi kinetik fluoresensi klorofil pada daun manggis paling lama dibagian pangkal yaitu 15,742 detik, sedangkan pada daun mengkudu waktu degradasi kinetik fluoresensi klorofil paling lama dibagian ujung daun yaitu 8,702 detik. Waktu degradasi yang dibutuhkan daun manggis lebih lama dibandingkan dengan daun mengkudu.\ud Kata kunci: Degradasi kinetik, Fluoresensi, Fotoluminesensi, Klorofil.\ud ABSTRACT\ud Study of degradation kinetic chlorophyll on the leaves (Garcinia Mangostana L and Morinda citrifolia L) has been investigated by using the fluorescence method. This aimes of this study are to detection of the chlorophyll and determine time of degradation kinetic chlorophyll used spectrometer HR2000 at 420 nanometer with fluorescence laser method. Sample used Garcinia Mangostana leaf and morinda citrifolia leaf. This measurement done in three part, namely the base leaves, the middle and the end of the leaves. The result of research obtained that fluorescence emission spectrum chlorophyll of Garcinia Mangostana leaf and Morinda Citrifolia leaf for every sample being in the wavelength from distance 679-735 nanometer, mean that the chlorophyll is absorb red spectrum. Time of degradation kinetic fluorescence of chlorophyll on Garcinia Mangostana leaf most long at the base of the 15,742 seconds while in the leaf of Morinda Citrifolia, time of degradation kinetic fluorescence of chlorophyll is the longest at the and of the leaf that is 8,702 seconds. 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