Protective Effect of Cocoa Extract on Albino Mice Skin Tumor After Exposure lo 7,12 Dimethylbenz (0) Antrasena (DMBA) and 12-0-Tetradecanoylphorbol-13-Acetate (TPA)Malondyaldehyde (MDA) analysis and Histopathology

-Aims.Oxidative stress result in DNA destruction in the Induction cells. If this damage is irreversible. DNA will undergo mutation and may lead to cancer. One of cancer caused chemical and often use in experiment study is DMBA/TPA. Thus the chemical is utilized in this study to examine protective effect of cocoa extract on mice. Method. *10 al bino mice.age 6??9 weeks, weight 20-30 gr were used as sampel study. The animal were divided into ( groups with different treatment group I, is negative control which receive no treatment; group II is control group receiving DMBA/TPA; group III receiving act-ton, and group IV.V, and VI cach receiving cocoa extract of ZOO,400 iiud 800 ppm, respectively Group ll-Vt expose to DMBA three times a week wl:h every other day fashion in week 1. followed by application of 4pg TPA on week 2-12. All the animal were sacrificed 24 h alter ast exposure and samples were taken and proccess for MDA and histopathology examination. Result. MDA level in 4flfl and BOO ppm cocoa cxtroct groups were higher than control, while it is lower in 200 ppm group than in control. Anova result showed no signicant differences among control and cocoa groups (p> 0 05). Histopathology examination result on DMBA/TPA control, aceton and 200 ppm cocoa groups consist of severe dysplasia and squamous cell carcinoma, whereas in 400 and 800 ppm cocoa groups some samples exhihit mild dysplasia. Kruskall Wallis slarisrir.il analysis indicates significant differences among control and cocoa groups (pS 0.05) (.jnclusion.Topicaliy applied 400 and BOO ppm cocoa exhibit protective effect un cantrr formation with the best protection effect shown in concentration of 800 pp