Molecular characterization of chronic lymphocytic leukemia patients with a high number of losses in 13q14.

Ana Eugenia Rodríguez
Jose Ángel Hernández
Rocío Benito
Norma C Gutiérrez
Juan Luis García
María Hernández-Sánchez
Alberto Risueño
M Eugenia Sarasquete
Encarna Fermiñán
Rosa Fisac
Alfonso García de Coca
Guillermo Martín-Núñez
Natalia de Las Heras
Isabel Recio
Oliver Gutiérrez
Javier De Las Rivas
Marcos González
Jesús M Hernández-Rivas

Open link

DOI

10.1371/journal.pone.0048485

Publisher

Public Library of Science (PLoS)

Journal

issn:1932-6203

Abstract

BACKGROUND: Patients with chronic lymphocytic leukemia and 13q deletion as their only FISH abnormality could have a different outcome depending on the number of cells displaying this aberration. Thus, cases with a high number of 13q- cells (13q-H) had both shorter overall survival and time to first therapy. The goal of the study was to analyze the genetic profile of 13q-H patients. DESIGN AND METHODS: A total of 102 samples were studied, 32 of which served as a validation cohort and five were healthy donors. RESULTS: Chronic lymphocytic leukemia patients with higher percentages of 13q- cells (>80%) showed a different level of gene expression as compared to patients with lower percentages (<80%, 13q-L). This deregulation affected genes invol...