ABSTRACT: BACKGROUND: Because protonation affects the properties of almost all molecules in cells, intracellular pH (pHc) is usually assumed to be constant. In the model organism yeast, however, pHc changes in response to the presence of nutrients and varies during growth. Since small changes in pHc can lead to major changes in metabolism, signal transduction, and phenotype, we decided to analyze pHc control. RESULTS: Introducing a pH-sensitive reporter protein into the yeast deletion collection allowed quantitative genome-wide analysis of pHc in live, growing yeast cultures. pHc is robust towards gene deletion; no single gene mutation lead to a pHc of more than 0.3 units lower than that of wildtype. Correct pHc control required not only va...
The budding yeast Saccharomyces cerevisiae exhibits exquisite control of cellular size in response t...
Glucose is the preferred carbon source for most cell types and a major determinant of cell growth. I...
Title: Changes of intracellular pH in yeast cells under stress conditions Author: Radek Divín Depart...
Abstract Background Because protonation affects the p...
Background: Nearly all processes in living cells are pH dependent, which is why intracellular pH (pH...
Recent research further clarified the molecular mechanisms that link nutrient signaling and pH homeo...
Protons, the smallest and most ubiquitous of ions, are central to physiological processes. Transmemb...
Cytosolic pH (pHc) is a crucial parameter in the cell because it is highly dynamic and its changes a...
Protons, the smallest and most ubiquitous of ions, are central to physiological processes. Transmemb...
Protons, the smallest and most ubiquitous of ions, are central to physiological processes. Transmemb...
Title Genome-wide analysis of intracellular pH reveals quantitative control of cell division rate by...
An asymmetry in cytosolic pH between mother and daughter cells was reported to underlie cellular agi...
A hallmark of all primary and metastatic tumours is their high rate of glucose uptake and glycolysis...
The specific pH values of cellular compartments affect virtually all biochemical processes, includin...
The conserved protein kinase Sch9 is a central player in the nutrient-induced signaling network in y...
The budding yeast Saccharomyces cerevisiae exhibits exquisite control of cellular size in response t...
Glucose is the preferred carbon source for most cell types and a major determinant of cell growth. I...
Title: Changes of intracellular pH in yeast cells under stress conditions Author: Radek Divín Depart...
Abstract Background Because protonation affects the p...
Background: Nearly all processes in living cells are pH dependent, which is why intracellular pH (pH...
Recent research further clarified the molecular mechanisms that link nutrient signaling and pH homeo...
Protons, the smallest and most ubiquitous of ions, are central to physiological processes. Transmemb...
Cytosolic pH (pHc) is a crucial parameter in the cell because it is highly dynamic and its changes a...
Protons, the smallest and most ubiquitous of ions, are central to physiological processes. Transmemb...
Protons, the smallest and most ubiquitous of ions, are central to physiological processes. Transmemb...
Title Genome-wide analysis of intracellular pH reveals quantitative control of cell division rate by...
An asymmetry in cytosolic pH between mother and daughter cells was reported to underlie cellular agi...
A hallmark of all primary and metastatic tumours is their high rate of glucose uptake and glycolysis...
The specific pH values of cellular compartments affect virtually all biochemical processes, includin...
The conserved protein kinase Sch9 is a central player in the nutrient-induced signaling network in y...
The budding yeast Saccharomyces cerevisiae exhibits exquisite control of cellular size in response t...
Glucose is the preferred carbon source for most cell types and a major determinant of cell growth. I...
Title: Changes of intracellular pH in yeast cells under stress conditions Author: Radek Divín Depart...