Functional proteomics analysis of transcription factor networks in erythroid cells

textabstractPost translational modifications (PTMs) are critical for regulating the function of many proteins. PTMs are widespread in eukaryotic cells and catalysed by specific enzymes. An important PTM is arginine methylation which is involved in many cellular processes, such as RNA metabolism, DNA damage repair, DNA replication and transcriptional regulation. Arginine methylation is catalysed by a family of enzymes called protein arginine methyltransferases (PRMTs). PRMT1 is ubiquitously expressed and is responsible for the majority of total protein arginine methylation activity in mammalian cells. The focus of the research described in this thesis is the isolation of Prmt1 associating proteins and the subsequent characterization of Chromatin target of Prmt1 (Chtop), one of the identified interactors