A Pseudomonas putida S12 strain was constructed that is able to convert glucose to p-coumarate via the central metabolite l-tyrosine. Efficient production was hampered by product degradation, limited cellular L-tyrosine availability, and formation of the by-product cinnamate via L-phenylalanine. The production host was optimized by inactivation of fcs, the gene encoding the first enzyme in the p-coumarate degradation pathway in P. putida, followed by construction of a phenylalanine-auxotrophic mutant. These steps resulted in a P. putida S12 strain that showed dramatically enhanced production characteristics with controlled l-phenylalanine feeding. During fed-batch cultivation, 10 mM (1.7 g l-1) of p-coumarate was produced from glucose with ...
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to efficiently utilize the C1 c...
Background : Pseudomonas putida KT2440 is able to synthesize large amounts of medium-chain-length po...
The rising price of oil and impending deficit of fossil resources stimulate the development of alter...
A Pseudomonas putida S12 strain was constructed that efficiently produced thefine chemical cinnamic ...
Pseudomonas putida is a saprophytic bacterium with robust metabolisms and strong solvent tolerance m...
Pseudomonas putida is a highly solvent-resistant microorganism and useful chassis for the production...
Two solvent-tolerant Pseudomonas putida S12 strains, originally designed for phenol and p-coumarate ...
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to utilize xylose as a substrat...
Pseudomonas putida KT2440 is an emerging biomanufacturing host amenable for use with renewable carbo...
Microbial biocatalysis represents a promising alternative for the production of a variety of aromati...
Pseudomonas putida strain S12palB1 was constructed that produces p-hydroxybenzoate from renewable ca...
Abstract With the increasing need for microbial bioproduction to replace petrochemicals, it is criti...
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to utilize xylose as a substrat...
Exceeding planetary boundaries, and in particular climate change, is quickly forcing the global econ...
Background: The soil bacterium Pseudomonas putida is a promising platform for the production of indu...
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to efficiently utilize the C1 c...
Background : Pseudomonas putida KT2440 is able to synthesize large amounts of medium-chain-length po...
The rising price of oil and impending deficit of fossil resources stimulate the development of alter...
A Pseudomonas putida S12 strain was constructed that efficiently produced thefine chemical cinnamic ...
Pseudomonas putida is a saprophytic bacterium with robust metabolisms and strong solvent tolerance m...
Pseudomonas putida is a highly solvent-resistant microorganism and useful chassis for the production...
Two solvent-tolerant Pseudomonas putida S12 strains, originally designed for phenol and p-coumarate ...
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to utilize xylose as a substrat...
Pseudomonas putida KT2440 is an emerging biomanufacturing host amenable for use with renewable carbo...
Microbial biocatalysis represents a promising alternative for the production of a variety of aromati...
Pseudomonas putida strain S12palB1 was constructed that produces p-hydroxybenzoate from renewable ca...
Abstract With the increasing need for microbial bioproduction to replace petrochemicals, it is criti...
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to utilize xylose as a substrat...
Exceeding planetary boundaries, and in particular climate change, is quickly forcing the global econ...
Background: The soil bacterium Pseudomonas putida is a promising platform for the production of indu...
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to efficiently utilize the C1 c...
Background : Pseudomonas putida KT2440 is able to synthesize large amounts of medium-chain-length po...
The rising price of oil and impending deficit of fossil resources stimulate the development of alter...