Extraction and structural analysis of glycosaminoglycans from formalin-fixed, paraffin-embedded tissues
- Wijk, X.M. van
- Vallen, M.J.E.
- Westerlo, E.M.A. van de
- Oosterhof, A.
- Hao, W.
- Versteeg, E.M.M.
- Raben, J.
- Wismans, R.
- Smetsers, T.F.
- Dijkman, H.B.P.M.
- Schalkwijk, J.
- Kuppevelt, A.H.M.S.M. van
DOIAbstract
Glycosaminoglycans (GAGs) are long, anionic polysaccharides involved in many basic aspects of mammalian physiology and pathology. Here we describe a method to extract GAGs from formalin-fixed, paraffin-embedded tissues and found that they are structurally comparable with GAGs extracted from frozen tissues. We employed this method to structurally characterize GAGs in tissues, including laser-dissected layers of skin and pathological specimens. This method enables the use of the archival paraffin-embedded material for detailed (structural) analysis of GAGs
Add to ORKG