Expression and effect of inhibition of aminopeptidase-A during nephrogenesis.

  • Dijkman, H.B.P.M.
  • Assmann, K.J.M.
  • Steenbergen, E.
  • Wetzels, J.F.M.
Publication date
January 2006
Publisher
SAGE Publications

Abstract

Aminopeptidase-A (APA) is a metalloprotease that cleaves N-terminal aspartyl and glutamyl residues from peptides. Its best-known substrate is angiotensin II (Ang II), the most active compound of the renin-angiotensin system (RAS). The RAS is involved in renal development. Most components of the RAS system are expressed in the developing kidney. Thus far, APA has not been studied in detail. In the present study we have evaluated the expression of APA at the protein, mRNA, and enzyme activity (EA) level in the kidney during nephrogenesis. Furthermore, we have studied the effect of inhibiting APA EA by injection of anti-APA antibodies into 1-day-old mice. APA expression was observed from the comma stage onwards, predominantly in the developing...

Extracted data

We use cookies to provide a better user experience.