Add to ORKGA defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations. Artifact sources were polymerase errors, a mutational hot spot, and cloning of heteroduplexes and chimeras. These data may partially explain the high degree of microheterogeneity typical of sequence clusters detected in environmental clone librarie
The advent of next generation sequencing has coincided with a growth in interest in using these appr...
A new computer program, called Mallard, is presented for screening entire 16S rRNA gene libraries of...
The t and emly repeated human ribosomal RNA (rRNA) genes, with 160 copies per haploid genome, consti...
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experi...
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experi...
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experi...
Analyses of 16S rDNA from cultured organisms and clone libraries have set the pace in molecular micr...
Statistical methods have had wide application in molecular biology. Genetic mapping, physical mappin...
Multiple band artifacts are seen commonly in the analyses of short repetitive sequences, also known ...
<p>Lanes labeled 1 to 21 give examples of individual clones that were randomly picked out from the c...
Next-generation sequencing has increased the coverage of microbial diversity surveys by orders of ma...
Analysis of the 16S rRNA genes retrieved directly from different environments has proven to be a pow...
The major objective of this research was to provide appropriate sequences and assemble a DNA array o...
<p>The number of rDNA gene fragments were determined by real time quantitative PCR from a standard c...
Analysis of complex genomes includes characterization of complete large-insert genomic libraries com...
The advent of next generation sequencing has coincided with a growth in interest in using these appr...
A new computer program, called Mallard, is presented for screening entire 16S rRNA gene libraries of...
The t and emly repeated human ribosomal RNA (rRNA) genes, with 160 copies per haploid genome, consti...
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experi...
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experi...
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experi...
Analyses of 16S rDNA from cultured organisms and clone libraries have set the pace in molecular micr...
Statistical methods have had wide application in molecular biology. Genetic mapping, physical mappin...
Multiple band artifacts are seen commonly in the analyses of short repetitive sequences, also known ...
<p>Lanes labeled 1 to 21 give examples of individual clones that were randomly picked out from the c...
Next-generation sequencing has increased the coverage of microbial diversity surveys by orders of ma...
Analysis of the 16S rRNA genes retrieved directly from different environments has proven to be a pow...
The major objective of this research was to provide appropriate sequences and assemble a DNA array o...
<p>The number of rDNA gene fragments were determined by real time quantitative PCR from a standard c...
Analysis of complex genomes includes characterization of complete large-insert genomic libraries com...
The advent of next generation sequencing has coincided with a growth in interest in using these appr...
A new computer program, called Mallard, is presented for screening entire 16S rRNA gene libraries of...
The t and emly repeated human ribosomal RNA (rRNA) genes, with 160 copies per haploid genome, consti...