The comparative analysis of the phenolic compounds and antioxidant activity in the herbal materials of Pitched Restharrow (Ononis arvensis L.)

Research aim: To investigate different parts of plant Pitched Restharrow(Ononis arvensis L.) phenolic compounds, flavonoids and antioxidant, antichelating activity.Objectives: 1. To determine the total phenolic compounds in different parts of Pitched Restharrow of the herbal materials;2. To determine the amount of flavonoids in different parts of Pitched Restharrow of the herbal materials;3. To evaluate the antioxidant ant antichelating activity in different parts of Pitched Restharrow of the herbal materials;4. To carry out a comparative analysis of biologically active compounds for different parts of plants and to identify the optimal medicinal herbal material composition of Pitched Restharrow. Methodology: This research was carried out using Kaunas and Kėdainiai district 2014-2015m. herbal materials of Pitched Restharrow. Extraction method – ultrasound extraction. In this research was determined the total phenolics and flavonoids content, antioxidant and antichelating activity. Results and conclusions: In the herbal materials of the Pitched Restharrow the total amount of phenolics and flavonoids had spread over a wide range by the morphological parts of the plant.The largest amount of common phenolic compounds was accumulated in 2015m. in the flowers around Kaunas district (22,82±2,8 mg/g) and the least in stem around Kėdainiai district in 2015m. The highest amount of flavonoids was also found in the flowers around Kaunas district in 2015m (70±0,002 mg/g) and the lowest in stems around Kėdainiai district. The highest antioxidant activity was detected around Kaunas district in 2015m. (34,97–89%), the lowest around Kėdainiai district in 2015m. The study showed a strong chelating with Fe (II) ions (39,53-62,11%). The statistical analysis of results was observed a strong correlation between phenolics and antioxidant activity (0.99, p