Supercritical fluid extraction of sterols from Eichhornia crassipes biomass using pure and modified carbon dioxide: enhancement of stigmasterol yield and extract concentration

The supercritical extraction of stalks and leaves of Eichhornia crassipes was carried out using pure andmodified carbon dioxide. Preliminary results evidenced that the influence of temperature is weakerthan pressure and ethanol content, and thus a design of experiments was performed in the rangesof 200–300 bar and 0.0–5.0 wt.% ethanol. The optima conditions for total extraction yield ( Total) were250–300 bar and 5.0 wt.% of cosolvent, while for sterols yield ( TotalSterol) they were 300 bar and 2.5 wt.%.The latter conditions were also the most favorable to maximize the concentration of total sterols in theextracts (CTotalSterol), particularly stigmasterol (CStigm), the most abundant one. For the referred optima, Total= 1.25 wt.%, TotalSterol= 0.35 wt.%, CTotalSterol= 38.26 wt.%, and CStigm= 26.35 wt . % . This study showedalso that the individual trends of stigmasterol and cholesterol were closer to the total sterols fractionbehavior than -sitosterol did, and also that the sterols concentration were higher in the first 2 h ofextraction. In the whole, this article provides a pertinent contribution for the valorization of E. crassipesbiomass through the production of natural extracts with high contents of sterols, especially stigmasterol,mainly if one takes into account it is classified as the most problematic invasive plant worldwide