The effect of supercritical fluid extraction parameters on the Swietenia mahagoni seed oil extraction and its cytotoxic properties

In this study, the influence of supercritical extraction parameter on the oil extraction of Mahogany (Swietenia mahagoni) was investigated. Supercritical extraction was carried out using the operation pressures of 20, 25 and 30 MPa, and temperature was varied in 40, 50 and 60°C. The parameter used for CO2 flow rate was constant, 2 mL/min, with extraction time of 180 min by using matrix particle size of 0.75 mm. Then, the extracted oils were tested for cytotoxic activity and properties. MTT assay was used as in vitro study to investigate the cytotoxic properties of the extracted on human fibroblast cells (HSF 1184). The study reveals that the extraction yield depends on the pressure and temperature. There is a significant difference of 40, 50 and 60°C at different pressures, whereby increasing pressure leads to significant increase extraction yields. MTT assay results indicate that all the extracts under different extraction parameters are non-cytotoxic. The results confirmed that supercritical extraction could be a promising technique to produce high quality botanicals extracts, free of solvent and non-cytotoxic with significant cost savings