Total Fenolat Dan Flavonoid Dari Ekstrak Dan Fraksi Daun Kumis Kucing (Orthosiphon Stamineus B.) Jawa Tengah Serta Aktivitas Antioksidannya

---Orthosiphon stamineus B (Lamiaceae) is a popular medicinal herb in South-east Asia, it was originated in Java Island and is well known by name of “Kumis Kucing.”. The biological activityof this plant, especially the antioxidant activity, is caused by the presence of compounds from phenolic group, especially come from flavonoid compounds. Therefore, the aim of this research is to determine total phenolic, total flavonoid and antioxidant activity analysis of methanol extract and its fractions of Orthosiphon stamineus B from Indonesia, especially Bandungan, Kabupaten Semarang, Jawa Tengah. The method of this research is consists of dried leaf extraction by methanolic, partition with solvent gradient, analysis of total phenolic and flavonoid content and antioxidant activity analysis. The total phenolic content analyzed by Folin-Ciocalteau method, total flavonoid content analyzed by Rohman method and antioxidantactivity analyzed by DPPH. radical scavenging method. The result showed that the highest phenolic and flavonoid content is in the ethyl acetate extract with value 559 mg gallic acid equivalent/g extract and 3550mg quercetin equivalent/g extract, respectively. The ethyl acetate has the strongest antioxidant activity with51.02 μg/mL of EC50 value. The finally antioxidant activity is shown in all extract of Indonesian Orthosiphonstamineus B can be used as antioxidant source