Analisis Cemaran Bakteri Coliform Dan Identifikasi Escherichia Coli Pada Air Isi Ulang Dari Depot Di Kota Manado

Water is a basic needs for human life, but potable water is getting hard to find. The high rates of environmental pollution affecting the availability of clean water. This study aims to analyze the bacterial contamination in drinking water refill from the depots with random sampling method from nine districts in the city of Manado. This research included the examination of Total Plate Count (TPC), examination of Coliform bacteria, and identification of Escherichia coli. The results of this study indicate that the all nine samples tested drinking water contained microbial contamination ranging from 1.6 x 103 to 2.9 x 104 colonies/mL. All samples contained coliform bacteria that do not qualify according to the Ministry of Health Regulation No. 492/MENKES/Per/IV/2010 which states 0 APM/100 mL sample. On the identification of Escherichia coli, first sample and ninth sample does not contain the bacteria Escherichia coli. While the other samples contained the bacterium Escherichia coli that do not qualify according to the Ministry of Health Regulation No. 492/MENKES/Per/IV/2010 are stated that in 100 mL of drinking water should not be contained of bacterium Escherichia coli