Research on the Antidepressive Mechanism of Chaiyuwendan Decoction Based on the Endogenous Cannabinoids system

目的： 1.观察柴郁温胆汤抗抑郁大鼠模型的药理效应； 2.从内源性大麻素系统探讨柴郁温胆汤抗抑郁的作用机制。 方法： 实验分两部分进行。 1.柴郁温胆汤抗抑郁大鼠模型的药效学实验：实验采用慢性轻度不可预见性应激21天复制大鼠抑郁模型。实验动物分为正常组、模型组、柴郁温胆汤组及氟西汀组，给药21天。观察大鼠体重变化，根据旷场实验和糖水消耗实验观察抑郁模型大鼠的行为学变化；采用Elisa法检测大鼠血清中5-HT、DA、ACTH、CORT的含量；采用酶法检测血清中TG、T-CHO、HDL-C、LDL-C的含量及血清ALT、AST的活性。 2.基于内源性大麻素系统探讨柴郁温胆汤抗抑郁的作用...Objective： 1. Observe the pharmacological effect of using Chaiyuwendan Decoction to treat rats with depression; 2. To investigate the mechanism of action using Chaiyuwendan Decoction to improve the rat model of depression from Endogenous Cannabinoid System. Method： The experiment was divided into two parts. 1. Pharmacodynamics of using Chaiyuwendan Decoction to treat rats with depression: Exp...学位：医学硕士院系专业：医学院_中医内科学学号：2452013115355