Adriamycin chemoresistance enriched for CD133+ hepatocarcinoma stem cells by activating Smad3

化疗是晚期肝癌患者辅助治疗的重要方法之一，但其效果不理想，肿瘤耐药性的产生是重要原因之一。目前研究发现肿瘤干细胞与化疗耐药性产生密切相关。因此，研究肝癌化疗耐药与肝癌干细胞之间的相关性并阐明其耐药机制，可以为晚期肝癌的治疗寻找新的有效治疗靶点。本研究通过运用化疗药物阿霉素（ADM）梯度溶度作用Huh7.5.1肝癌细胞，诱导耐药细胞株Huh7.5.1/ADM的形成，通过检测比较Huh7.5.1/ADM与Huh7.5.1细胞之间表面标记物CD133+的表达差异及信号传导蛋白Smad3的表达变化，并测试运用Smad3特异性抑制剂SIS3作用后耐药细胞株CD133+表达的逆转情况，初步探索化疗是否可以...Cancer stem cells(CSCs) are a small subset of cancer cells,which are thought to be responsible for chemotherapy failure, tumor relapse and metastasis. CD133+ is reported as a CSC marker in Hepatocellular carcinoma (HCC). Some studies suggest that CSCs could be enriched by chemotherapy. However, the mechanism of chemotherapy regulating CSCs remains unclear. Therefore , we investigated whether drug ...学位：医学硕士院系专业：医学院_外科学学号：2452012115317