Establishment and Application of Cell Lines for the detection of dioxins

二噁英是一类常见的持久性有机污染物，广泛存在于环境中。毒性作用剂量极低并且易于在生物体内富集，对环境和人体健康都具有很大的危害。环境中的二噁英组分复杂，对分析技术的特异性和灵敏度要求都非常高。 国际上公认的检测二噁英的HRGC/HRMS标准方法需要复杂的前处理，且费用昂贵，而应用生物检测手段检测二噁英类污染物也有很大的优势。本研究主要利用二噁英类化合物进入生物体内后，作为芳香烃受体（Aryhydrocarbonreceptor，AhR）的配体，促进AhR入核与CYP1A基因上游启动子区域的二噁英物质响应元件（dioxin-responsiveelement，DRE）结合，从而诱导其表达这一毒...Dioxin is well known as a persistent organic pollutants(pollutants (POPs),they, they are widely present in the environment. They have very high toxicity and easy to accumμlate in vivo. Dioxins on the environment and human health are of great harm. The component of Dioxins in the environment is very complex and technical analysis of specificity and sensitivity requirements are very high. Internat...学位：理学硕士院系专业：生命科学学院_生物化学与分子生物学学号：2162013115257