Study on the microbial community structure, diversity and abundance in water-soil interface sediments on the watershed of three rivers

为了探讨无机盐分、养分及有机质矿化作用对微生物群落结构、多样性和功能微生物丰度影响，河流水土界面微生物群落结构和碳、氮、硫循环功能微生物种群的演替规律，本论文以九龙江、闽江和瓯江三江流域作为研究区域，并增加温室培养实验，对其中水土界面沉积物样品中的理化参数、微生物群落、多样性和功能微生物丰度进行测定；采用基因文库构建技术、变性梯度凝胶电泳技术（DGGE）和illumina高通量测序技术分析了样品中微生物群落结构及活动规律；利用实时荧光定量PCR（RT-PCR）技术对功能微生物进行定量，分析丰度在不同盐分区域的变化规律，进而阐述环境因素和有机质矿化作用对微生物群落结构、多样性及功能微生物丰度影响...To investigate the impactof salinity, nutrient and organic matter mineralization on microbial community structure, diversity and abundance of functional microbe, sixty-one surface sediment samples were collected on the whole watershed of Jiulongjiang River, Minjiang River and Oujiang River, China, and cultured experiments in green house were designed. Physicochemical parameters of the sediment sam...学位：理学硕士院系专业：生命科学学院_微生物学学号：2162010115237