Survey of viral diversity in the sediments of South China Sea

病毒是海洋生态系统中丰度最大，多样性最为丰富的微生物颗粒。海洋沉积物中的病毒丰度相比较海水中，要高1~2个数量级，并且其形态和基因型也更加多样。目前，人们对海洋沉积物中的病毒相关研究还比较少。为了增强对海洋沉积物中病毒的了解，本论文利用荧光显微镜法获得了珠江口沉积物以及南海北部和西部沉积物中病毒的丰度数据，并且通过病毒标记基因（g23&psbA）PCR扩增、克隆文库测序等分子技术获得了沉积物中T4类噬菌体和噬藻体类群的多样性信息。主要的研究结果： （1）荧光显微镜检测结果显示珠江口沉积物中病毒（4.49×108~1.17×109viruses/g）和原核生物（2.63~9.55×108cel...Virus is the most abundant and diverse “life-form” in marine. Viral abundance in marine sediment is higher than that in seawater by one or two order of magnitude, and viral morphology and genotype is also more diverse. So far, there are a few of researches that focus on sedimental virus. In order to better understand them, we sampled the sediments from Pearl River Estuary and northern and western ...学位：理学硕士院系专业：海洋与地球学院_海洋生物学学号：2232013115140