Magnetic Fluid Hyperthermia: focusing on the absorption power mechanisms and its influence for in vitro experiments

Resumen del trabajo presentado a la 9th International Conference on Fine Particle Magnetism, celebrada en Gaithersburg (USA) del 13 al 17 de junio de 2016.Magnetic Hyperthermia has received great attention in the last decade, being one of the most promising applications of magnetic nanoparticles in biomedicine. Despite several studies were performed and published, including in vitro, in vivo and clinical studies, a better comprehension of the physical mechanisms of power absorption are needed, especially when the nanoparticles are in the biological conditions. In this work, we focused in the design of magnetite nanoparticles looking for a controlled heating in vitro hyperthermia experiments. The experiments were perfonned in BV2 cell lineage with magnetite nanoparticles coated with DEXTRAN and mPEG2000 (Fig. 1, left). In order to understand deeply the results, an intense study on the mechanisms involved in the Power Absorption of nanoparticles in an alternating magnetic field was previously perfonned, together with the magnetic and morphological characterization of the nanoparticles. In addition, the toxicity and cell uptake of the nanoparticles with both coatings were studied for this cellular model. lnterestingly, we obtain cell death induced without an overheating (Temperature < 38 oC) after 6 hours, probably induced by cell stress promoted by the nanoparticles in the hyperthermia experiment. The SP A obtained from the experiments were interpreted and simulated in terms of our previous experimental and theoretical hyperthermia studies for ferrofluid.Peer Reviewe