In vitro peripheral blood mononuclear cells sensitivity to steroids and identification of biomarkers for predicting clinical response in pediatric idiopathic nephrotic syndrome

Idiopathic nephrotic syndrome (INS) is the most common pediatric glomerulopathy (annual incidence: 2.7 cases/100.000 subjects aged 0-14 years), with a prevalence of 16 per 100.000 pediatric subjects. The therapy with prednisone is able to induce remission in 85-90% of children at onset, however, about 50% of them show frequent relapses, with the use of high doses of steroids, which involve inevitable side effects. To date, the optimal dose of steroids to be used in the first episode of INS has not been defined: the identification of biomarkers able to predict the sensitivity to glucocorticoids (GC) a priori could provide a useful tool to improve INS treatment and may be useful for developing an individualized therapy in these patients. With this background, the aim of the thesis was to identify the cellular and molecular markers associated with and predictive of outcome in pediatric INS. For this purpose, it has been developed a pharmacodynamic assay, to predict the sensitivity to GC in vitro and to assess the possible association between the clinical and the in vitro response in pediatric patients with INS. Preliminary studies were carried out to evaluate the associations between the clinical or the in vitro response and the presence of different genetic polymorphisms, the levels of expression of the GC receptor and of other proteins involved in the mechanism of action of GC, as well as of the long non coding RNA GAS-5 that has been found to regulate GC response. Moreover, the plasma levels of cytokines at disease onset and after 4 weeks of prednisone treatment were also evaluated. The studies in this thesis have hypothesized several candidates to predict GC response in children with INS, the results, being based on non-invasive methods, could lead to a turnaround in current treatment in childhood INS and could be relevant also for other pediatric populations treated with steroids.Idiopathic nephrotic syndrome (INS) is the most common pediatric glomerulopathy (incidence: 2.7 cases/year/100.000 subjects aged 0-14 years), with a prevalence of 16 per 100.000 pediatric subjects. The therapy with prednisone is able to induce remission in 85-90% of children at onset, however between the sensitive patients, 50% shown frequent relapses, with the use of high doses of steroids, which involve inevitable side effects . To date, however, the optimal dose of steroids to be used in the first episode of INS has not been defined yet: the identification of biomarkers to predict the sensitivity to glucocorticoids (GC) a priori could provide a clearer picture in the treatment of INS and may be useful in developing an individualized therapy in these patients. With this background, the aim of the thesis was to identify the cellular and molecular markers associated with and / or predictive of outcome in pediatric INS. For this purpose, it has been developed a pharmacodynamic tests in vitro, to predict the sensitivity to GC and assess the possible associations between the clinical and in vitro response of pediatric patients with INS. The plasma levels of cytokines were also evaluated, in order to identify a biomarker useful in the prediction of GC response. Moreover, preliminary studies were carried out to evaluate the possible associations between the clinical and the in vitro response with the presence of different genetic polymorphisms, with the levels of expression of the GC receptor and the role of an long non coding RNA, and the mRNA expression levels of proteins involved in the mechanism of action of GC. The studies in this thesis have hypothesized several methods to predict GC response in children with INS, the results, being based on non-invasive methods, could really cause a turnaround in current treatment in childhood INS and could be relevant also for other pediatric populations