mRNA export from the nucleus depends on the ATPase activity of the DEAD-box protein Dbp5/DDX19. Although Dbp5 has measurable ATPase activity alone, several regulatory factors (e.g., RNA, nucleoporin proteins, and the endogenous small molecule InsP6) modulate catalytic activity in vitro and in vivo to facilitate mRNA export. An analysis of the intrinsic and regulator-activated Dbp5 ATPase cycle is necessary to define how these factors control Dbp5 and mRNA export. Here, we report a kinetic and equilibrium analysis of the Saccharomyces cerevisiae Dbp5 ATPase cycle, including the influence of RNA on Dbp5 activity. These data show that ATP binds Dbp5 weakly in rapid equilibrium with a binding affinity (KT~4 mM) comparable to the KM for steady-s...
DEAD-box ATPases constitute a very large protein family present in all cells, often in great abundan...
Eukaryotic cells have developed a fascinating mechanism to ensure that the genetic information is tr...
Dbp5 is a DEAD‐box protein essential for mRNA export from the nucleus in yeast. Here we report the i...
mRNA export from the nucleus depends on the ATPase activity of the DEAD-box protein Dbp5/DDX19. Alth...
AbstractmRNA export from the nucleus depends on the ATPase activity of the DEAD-box protein Dbp5/DDX...
The DEAD-box protein Dbp5 is essential for RNA export, which involves regulation by the nucleoporins...
Superfamily 1 (SF1) and superfamily 2 (SF2) RNA helicases are ubiquitous mRNA-protein (mRNP) remodel...
Dbp5, DDX19 in humans, is an essential DEAD-box protein involved in mRNA export, which has also been...
The DEAD-box RNA-helicase Dbp5/Rat8 is known for its function in nuclear mRNA export, where it displ...
Messenger RNA (mRNA) export involves the unidirectional passage of ribonucleoprotein particles (RNPs...
The export of mRNA out of the nucleus is a crucial step for eukaryotic gene expression. The export o...
Mss116 is a Saccharomyces cerevisiae mitochondrial DEAD-box RNA helicase protein that is essential f...
In eukaryotic cells, messenger RNAs (mRNAs) are synthesized in the nucleus and then exported to the ...
DEAD-box proteins are ATPase enzymes that destabilize and unwind duplex RNA. Quantitative knowledge ...
The mRNA nuclear export process is a tightly regulated biological system important for eukaryotic ge...
DEAD-box ATPases constitute a very large protein family present in all cells, often in great abundan...
Eukaryotic cells have developed a fascinating mechanism to ensure that the genetic information is tr...
Dbp5 is a DEAD‐box protein essential for mRNA export from the nucleus in yeast. Here we report the i...
mRNA export from the nucleus depends on the ATPase activity of the DEAD-box protein Dbp5/DDX19. Alth...
AbstractmRNA export from the nucleus depends on the ATPase activity of the DEAD-box protein Dbp5/DDX...
The DEAD-box protein Dbp5 is essential for RNA export, which involves regulation by the nucleoporins...
Superfamily 1 (SF1) and superfamily 2 (SF2) RNA helicases are ubiquitous mRNA-protein (mRNP) remodel...
Dbp5, DDX19 in humans, is an essential DEAD-box protein involved in mRNA export, which has also been...
The DEAD-box RNA-helicase Dbp5/Rat8 is known for its function in nuclear mRNA export, where it displ...
Messenger RNA (mRNA) export involves the unidirectional passage of ribonucleoprotein particles (RNPs...
The export of mRNA out of the nucleus is a crucial step for eukaryotic gene expression. The export o...
Mss116 is a Saccharomyces cerevisiae mitochondrial DEAD-box RNA helicase protein that is essential f...
In eukaryotic cells, messenger RNAs (mRNAs) are synthesized in the nucleus and then exported to the ...
DEAD-box proteins are ATPase enzymes that destabilize and unwind duplex RNA. Quantitative knowledge ...
The mRNA nuclear export process is a tightly regulated biological system important for eukaryotic ge...
DEAD-box ATPases constitute a very large protein family present in all cells, often in great abundan...
Eukaryotic cells have developed a fascinating mechanism to ensure that the genetic information is tr...
Dbp5 is a DEAD‐box protein essential for mRNA export from the nucleus in yeast. Here we report the i...