Construction and Rescue of a Functional Synthetic Baculovirus
- Shang, Yu
- Wang, Manli
- Xiao, Gengfu
- Wang, Xi
- Hou, Dianhai
- Pan, Kai
- Liu, Shurui
- Li, Jiang
- Wang, Jun
- Arif, Basil M.
- Vlak, Just M.
- Chen, Xinwen
- Wang, Hualin
- Deng, Fei
- Hu, Zhihong
DOIAbstract
Synthetic viruses provide a powerful platform to delve deeper into the nature and function of viruses as well as to engineer viruses with novel properties. So far, most synthetic viruses have been RNA viruses (<30 kb) and small DNA viruses, such as bacteriophage phiX174. Baculoviruses contain a large circular dsDNA genome of 80-180 kb and have been used as biocontrol agents and protein expression vectors. Here, we report on the first synthesis of a baculovirus based on the type species Autographa californica nucleopolyhedrovirus, AcMNPV, by a combination of PCR and transformation-associated recombination in yeast. The synthetic genome, designated AcMNPV-WIV-Syn1, is 145 299 bp comprising the complete genome of AcMNPV except for the hr4a loc...
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